However, faecal tests can be inconvenient, complex and expensive. All rights reserved.įaecal tests are widely applied for colorectal cancer (CRC) screening and considered for triaging symptomatic patients with suspected CRC. The results have proven high quality of collected samples for both cytological investigation and diagnostic biomarker analysis.
Cms mus2 oct 2015 update trial#
The method was very well accepted by trial participants. MUC2 measurement applied as a test for IBD detection provided sensitivity = 72.4% and specificity = 86.7%.Ī novel non-invasive method for collecting human colorectal mucus has been successfully tested. MUC2 levels were similar among all IBD-free individuals (control and IBS groups) and elevated in IBD patients (p < 0.001). Total protein values varied in all groups, being affected by fecal contamination. Cytology showed large numbers of well preserved inflammatory cells in IBD cases. 55% of the collected samples were free of fecal contamination. The novel sampling technique was assessed as 'good' or 'adequate' by 96% of study participants. Collected samples were examined cytologically and subjected to quantitative analysis for total protein and mucin 2 (MUC2). Samples rich in colorectal mucus were self-collected by swabbing the anal area immediately following defecation. We aimed to: a) test a novel method of non-invasive colorectal mucus sampling in a pilot clinical trial b) evaluate sampling method acceptance by study participants c) characterize the collected material cytologically d) assess feasibility of quantitative protein analysis in the samples.ġ41 Patients with IBD (58), IBS (50) and healthy controls (33) participated in the study. Colorectal mucus is the diagnostically informative element of stool samples, but its separation from stool is difficult. Non-invasive diagnosis of colorectal disease remains problematic, fecal biomarkers presenting the only current option.
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